Observation of culture particularly for Paramecium (permanent slide also to be observed) and identification of other micro-organisms with the aid of books and making their drawings.
Aim Preparation of protozoan culture and identification of Paramecium and other micro organisms.
> Take one litre of water from a fresh water body like pond, pool etc and boil it.
> To the boiling water, add a handful of finely cut hay and continue to boil for another ten minutes.
> The mixture is then allowed to cool and is kept aside for two days
> A small quantity of pond water is collected and added to the above culture. Since the pond water contains protozoans they start multiplying within four to five days.
> After five days, take a drop of the above culture on a clean slide and observe under compound microscope, under both low and high power.
Observation : A large number of Paramecia are seen along with other microorganisms.